The Healing Power of 528 Hz Music: A Comparative Analysis with 440 Hz

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Music has always been a powerful medium for evoking emotions and influencing the mood of listeners. Over the years, different frequencies of music have been studied for their potential effects on the human psyche and physiology. Among these, the solfeggio frequency, particularly 528 Hz, has garnered attention for its purported “healing” properties. But how does it compare to the internationally recognized standard tuning frequency of 440 Hz?

Understanding the Frequencies: 528 Hz vs. 440 Hz

Typically, the global standard for tuning musical instruments is set at 440 Hz. This frequency serves as the reference tone for tuning, and in this musical scale, there is no note that resonates at 528 Hz. However, music that is specifically tuned and composed to include the 528 Hz note is referred to as solfeggio frequency music.

The Study: Effects of 528 Hz on Human Physiology and Mood

A recent investigation delved into the potential effects of 528 Hz music on human participants. In this study, nine individuals were exposed to soothing piano music resonating at either 528 Hz or 440 Hz. The participants were seated beside a speaker, and the sole variable between the two musical conditions was the frequency.

Key findings from the study include:

  1. Oxytocin Levels: Post exposure to 528 Hz music, there was a significant surge in oxytocin levels in the participants. While 440 Hz music also led to an increase in oxytocin, the rise was not as pronounced as with 528 Hz.
  2. Cortisol Levels: Cortisol, often referred to as the “stress hormone,” showed a notable decrease 30 minutes after participants listened to 528 Hz music. On the other hand, 440 Hz music resulted in only a marginal reduction in cortisol levels after the same duration.
  3. Stress Reduction: Utilizing salivary biomarkers, electrocardiogram data, and mood state questionnaires, the study deduced that a mere five minutes of exposure to 528 Hz music could significantly reduce stress levels. This effect was not observed with 440 Hz music.


While the solfeggio frequency, especially 528 Hz, is often touted for its healing properties, empirical evidence like the study mentioned above provides a more nuanced understanding. The potential benefits of 528 Hz music are intriguing, but more extensive research is needed to draw definitive conclusions. In summary, the study indicated that individuals who listened to 528 Hz music experienced a more profound sense of relaxation and reduced stress compared to those who listened to 440 Hz music. However, it’s essential to note that the study had a limited sample size, which calls for further research to validate these findings.


Akimoto, K., Hu, A., Yamaguchi, T., & Kobayashi, H. (2018). Effect of 528 Hz Music on the Endocrine System and Autonomic Nervous System. Health, 10(9), 1159-1170. DOI: 10.4236/health.2018.109088

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